Nuclear Magnetic Resonance and Molecular Dynamics Simulation of the Interaction between Recognition Protein H7 of the Novel Influenza Virus H7N9 and Glycan Cell Surface Receptors
Identifieur interne : 000418 ( Main/Exploration ); précédent : 000417; suivant : 000419Nuclear Magnetic Resonance and Molecular Dynamics Simulation of the Interaction between Recognition Protein H7 of the Novel Influenza Virus H7N9 and Glycan Cell Surface Receptors
Auteurs : Eleonora Macchi [Italie] ; Timothy R. Rudd [Royaume-Uni] ; Rahul Raman [États-Unis] ; Ram Sasisekharan [États-Unis] ; Edwin A. Yates [Royaume-Uni] ; Annamaria Naggi [Italie] ; Marco Guerrini [Italie] ; Stefano Elli [Italie]Source :
- Biochemistry [ 0006-2960 ] ; 2016.
Abstract
Avian influenza A viruses, which can also propagate between humans, present serious pandemic threats, particularly in Asia. The specificity (selectivity) of interactions between the recognition protein hemagglutinin (HA) of the virus capsid and the glycoconjugates of host cells also contributes to the efficient spread of the virus by aerosol between humans. Some avian origin viruses, such as H1N1 (South Carolina 1918), have improved their selectivity for human receptors by mutation in the HA receptor binding site, to generate pandemic viruses. Molecular details and dynamics of glycan–HA interactions are of interest, both in predicting the pandemic potential of a new emerging strain and in searching for new antiviral drugs. Two complementary techniques, 1H saturation transfer difference (1H STD) nuclear magnetic resonance and molecular dynamics (MD) simulation, were applied to analyze the interaction of the new H7 (A/Anhui/1/13 H7N9) with LSTa [Neu5Ac
Url:
DOI: 10.1021/acs.biochem.6b00693
PubMed: 27933797
PubMed Central: 5559875
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000797
- to stream Pmc, to step Curation: 000797
- to stream Pmc, to step Checkpoint: 000298
- to stream Ncbi, to step Merge: 000D26
- to stream Ncbi, to step Curation: 000D26
- to stream Ncbi, to step Checkpoint: 000D26
- to stream Main, to step Merge: 000418
- to stream Main, to step Curation: 000418
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nuclear Magnetic Resonance and Molecular Dynamics Simulation of the Interaction between Recognition Protein H7 of the Novel Influenza Virus H7N9 and Glycan Cell Surface Receptors</title>
<author><name sortKey="Macchi, Eleonora" sort="Macchi, Eleonora" uniqKey="Macchi E" first="Eleonora" last="Macchi">Eleonora Macchi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rudd, Timothy R" sort="Rudd, Timothy R" uniqKey="Rudd T" first="Timothy R." last="Rudd">Timothy R. Rudd</name>
<affiliation wicri:level="1"><nlm:aff id="A2">National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, U.K</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG</wicri:regionArea>
<wicri:noRegion>Hertfordshire EN6 3QG</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Raman, Rahul" sort="Raman, Rahul" uniqKey="Raman R" first="Rahul" last="Raman">Rahul Raman</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139</wicri:regionArea>
<wicri:noRegion>Massachusetts 02139</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sasisekharan, Ram" sort="Sasisekharan, Ram" uniqKey="Sasisekharan R" first="Ram" last="Sasisekharan">Ram Sasisekharan</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139</wicri:regionArea>
<wicri:noRegion>Massachusetts 02139</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yates, Edwin A" sort="Yates, Edwin A" uniqKey="Yates E" first="Edwin A." last="Yates">Edwin A. Yates</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB</wicri:regionArea>
<wicri:noRegion>Liverpool L69 7ZB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Naggi, Annamaria" sort="Naggi, Annamaria" uniqKey="Naggi A" first="Annamaria" last="Naggi">Annamaria Naggi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guerrini, Marco" sort="Guerrini, Marco" uniqKey="Guerrini M" first="Marco" last="Guerrini">Marco Guerrini</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Elli, Stefano" sort="Elli, Stefano" uniqKey="Elli S" first="Stefano" last="Elli">Stefano Elli</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27933797</idno>
<idno type="pmc">5559875</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559875</idno>
<idno type="RBID">PMC:5559875</idno>
<idno type="doi">10.1021/acs.biochem.6b00693</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000797</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000797</idno>
<idno type="wicri:Area/Pmc/Curation">000797</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000797</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000298</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000298</idno>
<idno type="wicri:Area/Ncbi/Merge">000D26</idno>
<idno type="wicri:Area/Ncbi/Curation">000D26</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000D26</idno>
<idno type="wicri:doubleKey">0006-2960:2016:Macchi E:nuclear:magnetic:resonance</idno>
<idno type="wicri:Area/Main/Merge">000418</idno>
<idno type="wicri:Area/Main/Curation">000418</idno>
<idno type="wicri:Area/Main/Exploration">000418</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nuclear Magnetic Resonance and Molecular Dynamics Simulation of the Interaction between Recognition Protein H7 of the Novel Influenza Virus H7N9 and Glycan Cell Surface Receptors</title>
<author><name sortKey="Macchi, Eleonora" sort="Macchi, Eleonora" uniqKey="Macchi E" first="Eleonora" last="Macchi">Eleonora Macchi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rudd, Timothy R" sort="Rudd, Timothy R" uniqKey="Rudd T" first="Timothy R." last="Rudd">Timothy R. Rudd</name>
<affiliation wicri:level="1"><nlm:aff id="A2">National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, U.K</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG</wicri:regionArea>
<wicri:noRegion>Hertfordshire EN6 3QG</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Raman, Rahul" sort="Raman, Rahul" uniqKey="Raman R" first="Rahul" last="Raman">Rahul Raman</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139</wicri:regionArea>
<wicri:noRegion>Massachusetts 02139</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sasisekharan, Ram" sort="Sasisekharan, Ram" uniqKey="Sasisekharan R" first="Ram" last="Sasisekharan">Ram Sasisekharan</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139</wicri:regionArea>
<wicri:noRegion>Massachusetts 02139</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yates, Edwin A" sort="Yates, Edwin A" uniqKey="Yates E" first="Edwin A." last="Yates">Edwin A. Yates</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB</wicri:regionArea>
<wicri:noRegion>Liverpool L69 7ZB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Naggi, Annamaria" sort="Naggi, Annamaria" uniqKey="Naggi A" first="Annamaria" last="Naggi">Annamaria Naggi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guerrini, Marco" sort="Guerrini, Marco" uniqKey="Guerrini M" first="Marco" last="Guerrini">Marco Guerrini</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Elli, Stefano" sort="Elli, Stefano" uniqKey="Elli S" first="Stefano" last="Elli">Stefano Elli</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto di Ricerche Chimiche e Biochimiche “G. Ronzoni”, Via Giuseppe Colombo 81, 20133 Milano</wicri:regionArea>
<wicri:noRegion>20133 Milano</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochemistry</title>
<idno type="ISSN">0006-2960</idno>
<idno type="eISSN">1520-4995</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Avian influenza A viruses, which can also propagate between humans, present serious pandemic threats, particularly in Asia. The specificity (selectivity) of interactions between the recognition protein hemagglutinin (HA) of the virus capsid and the glycoconjugates of host cells also contributes to the efficient spread of the virus by aerosol between humans. Some avian origin viruses, such as H1N1 (South Carolina 1918), have improved their selectivity for human receptors by mutation in the HA receptor binding site, to generate pandemic viruses. Molecular details and dynamics of glycan–HA interactions are of interest, both in predicting the pandemic potential of a new emerging strain and in searching for new antiviral drugs. Two complementary techniques, <sup>1</sup>
H saturation transfer difference (<sup>1</sup>
H STD) nuclear magnetic resonance and molecular dynamics (MD) simulation, were applied to analyze the interaction of the new H7 (A/Anhui/1/13 H7N9) with LSTa [Neu5Ac <italic>α</italic>
(2→3) Gal <italic>β</italic>
(1→3) GlcNAc <italic>β</italic>
(1→3) Gal <italic>β</italic>
(1→4) Glc] and LSTc [Neu5Ac <italic>α</italic>
(2→6) Gal <italic>β</italic>
(1→4) GlcNAc <italic>β</italic>
(1→3) Gal <italic>β</italic>
(1→4) Glc] pentasaccharides, models of avian and human receptor glycans. Their interactions with H7 were analyzed for the first time using <sup>1</sup>
H STD and MD, revealing structural and dynamic behavior that could not be obtained from crystal structures, and contributing to glycan–HA specificity. This highlighted aspects that could affect glycan–HA recognition, including the mutation H7 G228S, which increases H2 and H3 specificity for the human receptor. Finally, interactions between LSTc and H7 were compared with those between LSTc and H1 of H1N1 (South Carolina 1918), contributing to our understanding of the recognition ability of HAs.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Italie</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="Italie"><noRegion><name sortKey="Macchi, Eleonora" sort="Macchi, Eleonora" uniqKey="Macchi E" first="Eleonora" last="Macchi">Eleonora Macchi</name>
</noRegion>
<name sortKey="Elli, Stefano" sort="Elli, Stefano" uniqKey="Elli S" first="Stefano" last="Elli">Stefano Elli</name>
<name sortKey="Guerrini, Marco" sort="Guerrini, Marco" uniqKey="Guerrini M" first="Marco" last="Guerrini">Marco Guerrini</name>
<name sortKey="Naggi, Annamaria" sort="Naggi, Annamaria" uniqKey="Naggi A" first="Annamaria" last="Naggi">Annamaria Naggi</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Rudd, Timothy R" sort="Rudd, Timothy R" uniqKey="Rudd T" first="Timothy R." last="Rudd">Timothy R. Rudd</name>
</noRegion>
<name sortKey="Yates, Edwin A" sort="Yates, Edwin A" uniqKey="Yates E" first="Edwin A." last="Yates">Edwin A. Yates</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Raman, Rahul" sort="Raman, Rahul" uniqKey="Raman R" first="Rahul" last="Raman">Rahul Raman</name>
</noRegion>
<name sortKey="Sasisekharan, Ram" sort="Sasisekharan, Ram" uniqKey="Sasisekharan R" first="Ram" last="Sasisekharan">Ram Sasisekharan</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000418 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000418 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:5559875 |texte= Nuclear Magnetic Resonance and Molecular Dynamics Simulation of the Interaction between Recognition Protein H7 of the Novel Influenza Virus H7N9 and Glycan Cell Surface Receptors }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27933797" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |